Aboriginal elder Bill Smith performed a smoking ceremony at Enterprise Park on Saturday.
The ceremony was part of a protest organised by the Hunter Region Concerned Citizens, a group in support of keeping the rail in Newcastle.
The rail is expected to get truncated on December 26.
Over eighty people gathered to see what Mr Smith described as a ceremony about "cleansing, strengthening and coming together."
"This Rail Line is a safe passage for all the People and belongs to all of us. We must stand as one and join hands regardless of race, colour or creed to defend this important link, and keep it in place for future generations.”
Mr Smith’s son, Edward, played the didgeridoo as protesters moved through the smoke from burning gum leaves.
Richard Green, Deputy Chair of the Awabakal Land Council, who also attended the protest said “The Aboriginal Community has not been consulted about what is happening to this traditional and spiritual land."
Greens' councillors for Newcastle Michael Osborne and Therese Doyle were among those present.